A sensitive, selective, precise, accurate high-performance thin layer chromatography (HPTLC) method was developed and validated for analysis of Flupentixol and Melitracen both as a bulk drug and in formulations. The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was consisted of ethyl acetate : methanol : ammonia in a proportion of (7.0:3.0:0.1) (v/v/v). Densitometric analysis of Flupentixol and Melitracen was carried out in the absorbance mode at 265 nm.This system was found to give compact spots for Flupentixol and Melitracen RF value of (0.73 ± 0.01 and 0.59 ± 0.01 for six replicates). The method was validated for linearity, precision, accuracy, specificity, LOD, LOQ, robustness, and ruggedness. Response was a linear function of Flupentixol concentration in the range of 20–120 ng/spot with significantly high value of correlation coefficient r2 = 0.99945 and Melitracen 100-600 ng/spot with significantly high value of correlation coefficient r2 = 0.99931. The limit of detection and quantification for Flupentixol were 7.04 and 21.22 ng/spot, respectively and for Melitracen limit of detection and quantification were 9.3 and 28.38 ng/spot respectively. The drug content for marketed formulation was found to be 98.3 % for Melitracen & 101.36 % Flupentixol for six replicate determinations. The established method enabled accurate, precise, and rapid analysis of Flupentixol and Melitracen in bulk as well as pharmaceutical formulation.
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